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You are here : PCR / DNA Amplification » Polymerases » m-Anti-Taq

m-Anti-Taq

Enhancer for Polymerase reactions

Cat.-no Description Amount Price € Shop
 S131   Maximo m-Anti Taq (AHO3)  100 µg      65.00  add
 S132   Maximo m-Anti Taq (AHO3)  500 µg    270.00  add
 S131-5M   Maximo m-Anti Taq (8C1, 5 mg/ml)  100 µg      65.00  add
 S132-5M   Maximo m-Anti Taq (8C1, 5 mg/ml)  500 µg    270.00  add

m-Anti-Taq: Order/request by E-mail:
m-Anti-Taq: Datasheet
m-Anti-Taq: Deutsche Beschreibung


Features:
- Anti-Taq inhibits the reaction of Polymerases at room temperature
- Ideal as enhancer for PCR reactions

Application:
Polymerase detergent/enhancer for:
- High specific amplification
- Multiplex amplification
- High sensitivity applications
- Low-copy number PCR

Unit definition:
One unit is defined as the amount required to blocks 50% activity of 1 µg of  Polymerase

Note:
The ratio units/mg of polymerase varies (up  to factor of 10). The binding rate Anti-Taq depends strongly of the polymerization region (active epitopes) of the polymerase.  The optimized mixture has to be found in empiric test and for every new lot of polymerase, again.

Storage Buffer:
20mM Tris-HCl (pH 7.0, at 22oC);50 mM KCl;0.1mM EDTA; 50% glycerol

Concentration: 2-10 mg/ml

Units/mg-ratio:
2300 units of the specific polymerase activity are equal to 1 mg of antibodies

QC-Test:
> 95% of protein in SDS electrophoresis in 15% PAAG

Storage: at – 20°C

Transportation: at room temperature or with blue ice

Related products:

 m-Superhot Taq DNA Polymerase
 dNTP-mix and dNTP sets
 DNA-Markers


 

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Overview

Overview Polymerases:

DFS-Taq DNA Polymerase DNA-free
Tag DNA Polymerase (DNA free) is a highly purified polymerase (DNA-free) especially for PCR reactions where freedom from endogenous template (i.e., E. coli DNA) is essential
Taq DNA Polymerase
Maximo Tag DNA Polymerase is a highly purified polymerase for routine amplification
Taq DNA Polymerase 2X-preMix
Maximo Tag DNA as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR
Taq DNA Polymerase blue ready-to-load
Maximo Tag-Blue DNA is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed!
m-Superhot Taq DNA pol.
M-Superhot Taq DNA Polymerase is developed to enhance the specificity, sensitivity and yield of DNA amplification. The enzyme provides a convenient setting up at room temperature
m-Anti-Taq
Mouse Monoclonal Antibody - Enhancer for Polymerase reactions
p-Superhot Taq DNA pol.
p-Superhot Taq DNA Polymerase is developed to enhance the specificity and yield of DNA amplification. The polymerase provides a convenient setting up at room temperature because of blocking the the enzyme with optimized mix of inhibitors
Pfu/Psp DNA Polymerase
Pfu DNA polymerase generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragments are blunt-ended for direct ligation
Pfu/Psp red DNA Polymerase RTL
Pfu DNA polymerase (RTU=READY-TO-LOAD) is a convenient Mixture of proof-reading Enzyme, red dye and loading buffer. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Pfu/Psp DNA Polymerase 2X-preMix
Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Tth DNA Polymerase
Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium.


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