Ready-to-use lyophilized PCR-Master Mix (Hot-Start) in 8-tubes-strips

Description: 
Maximo Dry Master-Mix is optimized and ready-to-use mixture of all components for a successful PCR. Only diluent-solution your primers and DNA Template has to be added. 
Maximo Dry Master-Mix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

List of components:  
12x8 lyophilized PCR-Tubes (0,2ml) "flat cap", PCR-diluent, PCR-oil (the thin PCR-Tubes fit to all cyclers were 0,2 ml Tubes with falt caps are required: ABI; Bio-Rad etc.)

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74°C

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: at room temperature

Storage: for long term storage at 4-8°C, short term: RT

Usage:  

Components	Volume per reaction
1 Tube of Maximo Dry Mastermix
PCR Diluent	10 µl
Up-stream primer (0.1-0.5 mM)	5 µl
Down-stream primer (0.1-0.5 mM)	5 µl
Template DNA	0.1-15 ng/ml plasmid DNA  1-10 µg/ml genomic DNA  (2-10 µl)
Sterile dest. Water (molecular grade)	up to 25 µl total reaction volume  if necessary: overlay with PCR-oil

Note: 
- vortex all solutions and spin down carefully before using


General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	2-4 min	94-95°C
25-30 Cycles:  Denaturation  Annealing  Extension	10-25 sec  10-25 sec  60 sec	94-95°C  45-70°C  68-72°C per 1kb
Final extension	5 min	68-72°C

Note: 
- In few cases the annealing temperature should be reduced with 2-3 °C 
- Post PCR: There is no need to add stop-solution or stain in the samples before loading on the gel.
  Just load 5-10 µl of the mix on the agarose gel.


Related Products:
Diagnostic PCR-Master Mixes - freeze dryed



 

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