PCR-detection-Master Mix for Agarose Gels - ready-to-use lyophilised PCR Master Mix

PCR Detection-Test-systems for the identification of infection diseases provide a convenient analysis of infection agents presence in biological samples.

All reagents (Taq DNA polymerase, dNTPs, specific primers, salts and stabilizers) are lyophilized in PCR tubes (0,2 ml), only sample to be analyzed and dilution buffer should be added before running the PCR.

The PCR-Test-System contains:
- 80 DNA test tubes
- 10 positive controls 
- 10 negative controls 
- in total: 100 tubes with lyophilized reagents (1 tube – 1 reaction)
- Specific dilution buffer


PCR-detection test system: Datasheet
PCR-Detektions Testsystem: Deutsche Beschreibung

Procedure (total volume 20 µl):
Preparing of DNA:
Choose any purification kit for preparing the DNA of the following sources:
- blood, tissue, cells suspension and other samples with high concentration of proteins, polysaccharides and DNA
- Saliva, urine, tears, mucus and other samples with low concentrations of DNA, proteins, polysaccharides and fats;

Preparing the reaction-mixture:
- open the 0,2 ml PCR Tube with lyophilised PCR Master Mix
- add max. 10 µl of DNA from bio-sample
- add max. 10 µl of dilution-buffer
- optional: add dest. Water (mol-bio grade) up to 20 µl
- vortex the reaction mixture extensive

Cycler-Protocol:

Step	Time	Temperature
Initial denaturation	2-3 min	95°C
45 Cycles:  Denaturation  Annealing  Extension	20-25 sec  20-40 sec  60 sec	95°C  58-62°C   *1)  74°C
Final extension	2-3 min	74°C

*1) depending on the test (see manual)

Analysis: on Agarose-gel

Please note: The Kits are for R&D only - no CE-Certification

Transport: at room-temperature (15 - 30°C)

Storage: recommended to store at +2 - +15°C for long term; for short time at room-temperature

Related products:
Agarose for Gelelectrophoresis
DNA Ladders




 

Product code	Description	Short-cut	Temp.	Ampl.size (bp)	Price €	shop
G10	Adeno-associated virus, type 2	AAV-2	58°C	450	399.00	add
G20	Adenovirus, general	AdV gen	56°C	272	399.00	add
G30	Adenovirus, genotypes	AdV types	60°C	272	399.00	add
G40	Aleutian mink disease parvovirus	AMDV	58°C	298	399.00	add
G50	Aspergilus fumigatus	Afu	58°C	456	399.00	add
G60	Babesia canis	Bca	58°C	359	399.00	add
G70	Babesia divergens	Bdi	58°C	359	399.00	add
G80	Babesia equi	Beq	58°C	362	399.00	add
G90	Babesia microti	Bmi	58°C	362	399.00	add
G100	Babesia spp.	Bab	58°C	359	399.00	add
G110	Bacillus anthracis	Ban	58°C	320	399.00	add
G120	Bacteroides fragilis	Bfr	58°C	535	399.00	add
G130	Bordetella pertussis	Bpe	58°C	279	399.00	add
G140	Borrelia afzelii	Baf	58°C	445	399.00	add
G150	Borrelia burgdorferi	Bbu	58°C	445	399.00	add
G160	Borrelia garinii	Bga	58°C	445	399.00	add
G170	Borrelia spp.(burgdof.+garinii+afzelii)	Bor	58°C	445	399.00	add
G180	Bovine herpes virus,I type	BHV I	58°C	355	399.00	add
G190	Bovine immunodeficiency virus	proBIV	58°C	382	399.00	add
G200	Bovine leukemia virus (gag)	proBLV	58°C	347	399.00	add
G210	Brucella melitensis.	Bme.	62°C	442	399.00	add
G220	Campylobacter jejuni	Cje	58°C	364	399.00	add
G230	Candida albicans	Cal	58°C	490	399.00	add
G240	Candida glabrata	Cgl	58°C	295	399.00	add
G250	Chlamydia pneumoniae	Cpn.	58°C	448	399.00	add
G260	Chlamydia psittaci	Cps.	58°C	356	399.00	add
G270	Chlamydia spp.	Chl.	58°C	560	399.00	add
G280	Chlamydia trachomatis	Ctr	58°C	410	399.00	add
G290	Clostridium difficile	Cdi	58°C	517	399.00	add
G300	Cryptococcus neoformans	Cne	58°C	307	399.00	add
G310	Cryptosporidium parvum	Cpa	58°C	475	399.00	add
G320	Ehrlichia murris/Yamaguchi	Emu	58°C	223	399.00	add
G330	Ehrlichia spp.	Ehr	58°C	337	399.00	add
G340	Ehrlichia(Anaplasma) phagocytophilum	Epf	58°C	227	399.00	add
G350	Entamoeba histolitica	Ehi	58°C	887	399.00	add
G360	Enterococcus faecalis	Efal	58°C	499	399.00	add
G370	Enterococcus faecium	Efam	58°C	541	399.00	add
G380	Epstein-Barr virus	EBV	58°C	558	399.00	add
G390	Erythromycin resistance gene, ermF	ermF	58°C	492	399.00	add
G400	Escherichia coli,CFT07, uropathogenic	Eco	58°C	606	399.00	add
G410	Francisella tularensis	Ftu	58°C	333	399.00	add
G420	Gardnerella vaginalis	Gva.	58°C	424	399.00	add
G430	Giardia lamblia	Gla	58°C	318	399.00	add
G440	Haemophilus influenzae	Hin	58°C	381	399.00	add
G450	Helicobacter pylori (cagA)	Hpy (cagA)	58°C	682	399.00	add
G460	Helicobacter pylori (ure)	Hpy (ure)	58°C	318	399.00	add
G470	Helicobacter pylori (vacA)	Hpy (vacA)	58°C	565	399.00	add
G480	Hepatitis B virus	HBV	58°C	473	399.00	add
G490	Herpes simplex virus 1 type	HSV 1	58°C	331	399.00	add
G500	Herpes simplex virus 1/2 types	HSV 1/2	58°C	360	399.00	add
G510	Herpes simplex virus 2 type	HSV 2	58°C	432	399.00	add
G520	Human papillomavirus, low risk	HPV l.r.	58°C	450	399.00	add
G530	Human cytomegalovirus	HCMV	58°C	451	399.00	add
G540	Human herpes virus 6 type	HHV 6	58°C	371	399.00	add
G550	Human herpes virus 7 type	HHV 7	58°C	437	399.00	add
G560	Human herpes virus 8 type	HHV 8	58°C	272	399.00	add
G570	Human immunodeficiency virus 1	proHIV I	58°C	376	399.00	add
G580	Human papillomavirus, 16 type	HPV 16	60°C	692	399.00	add
G590	Human papillomavirus, 16/18 types	HPV16/18	60°C	692	399.00	add
G600	Human papillomavirus, 18 type	HPV 18	60°C	692	399.00	add
G610	Human papillomavirus, general	HPVgen.	56°C	450	399.00	add
G620	Human papillomavirus, high risk	HPV h.r.	58°C	450	399.00	add
G630	Human papillomavirus, high risk-plus	HPV h.r.+	58°C	692	399.00	add
G640	Human papillomavirus,genotyping	HPV typing	60°C	450	399.00	add
G650	HumanT-lymphocytotropic virus 1/II	proHTLV I/II	60°C	598	399.00	add
G660	Klebsiella pneumoniae	Kpn	58°C	541	399.00	add
G670	Lactobacillus spp.	Lac	58°C	239	399.00	add
G680	Legionella pneumophila	Lpn	58°C	511	399.00	add
G690	Leptospira interrogans	Lin.	58°C	668	399.00	add
G700	Leptospira spp. (pathogenic serovars)	Lep.	58°C	423	399.00	add
G710	Listeria monocytogenes	Lmo	58°C	226	399.00	add
G720	Listeria spp.	Lis	58°C	429	399.00	add
G730	Mobiluncus curtisii	Mcu	58°C	224	399.00	add
G740	Moraxella catarrhalis	Mca	58°C	550	399.00	add
G750	Morganella morganii	Mmo	58°C	356	399.00	add
G760	Mycobacterium avium,subspp paratubercul.	Mav	62°C	268	399.00	add
G770	Mycobacterium intracellulare	Min	62°C	234	399.00	add
G780	Mycobacterium tuberculosis	Mtu.	62°C	222	399.00	add
G790	Mycobacterium tuberculosis+bovis	M(tu+bo)	62°C	211	399.00	add
G800	Mycoplasma hominis	Mho.	58°C	282	399.00	add
G810	Mycoplasma pneumoniae	Mpn.	58°C	316	399.00	add
G820	Mycoplasma fermentans	Mfe	58°C	700	399.00	add
G830	Mycoplasma genitalium	Mge	58°C	538	399.00	add
G840	Mycoplasma penetrans	Mpe	58°C	318	399.00	add
G850	Mycoplasma spp.(Mge+Mho+Mpn+Mfe+Mpe)	Myc	58°C	316	399.00	add
G860	Neisseria gonorrhoea	Ngo	58°C	591	399.00	add
G870	Parvovirus B19	PV B19	58°C	743	399.00	add
G880	Peotostreptococcus anaerobius	Pan	58°C	396	399.00	add
G890	Plasmodium falciparum	Pfa	58°C	319	399.00	add
G900	Plasmodium malariae	Pma	58°C	307	399.00	add
G910	Plasmodium ovale	Pov	58°C	312	399.00	add
G920	Plasmodium spp.(Pfa+Pvi+Pov+Pma)	Pla	58°C	319	399.00	add
G930	Plasmodium vivax	Pvi	58°C	310	399.00	add
G940	Pneumocystis carinii	Pca.	58°C	396	399.00	add
G950	Poliomavirusis (JCV, BKV, SV40)	JCV,BKV,SV	58°C	232	399.00	add
G960	Prevotella bivia	Pbi	58°C	279	399.00	add
G970	Proteus mirabilis	Pmi	58°C	448	399.00	add
G980	Pseudomonas aeruginosa	Pae	58°C	254	399.00	add
G990	Rickettsia spp.	Ric	58°C	259	399.00	add
G1000	SEN virus D	SENV-D	58°C	346	399.00	add
G1010	SEN virus D/H	SENV-D/H	58°C	346	399.00	add
G1020	SEN virus H	SENV-H	58°C	346	399.00	add
G1030	Streptococcus agalactiae	Sag.	58°C	583	399.00	add
G1040	Streptococcus pneumoniae	Spn	58°C	567	399.00	add
G1050	Streptococcus pyogenes	Spy	58°C	609	399.00	add
G1060	Tetracycline resistance gene, tetM	tetM	58°C	430	399.00	add
G1070	Tetracycline resistance gene, tetQ	tetQ	58°C	392	399.00	add
G1080	Toxoplasma gondii	Tgo	58°C	523	399.00	add
G1090	Transfusion transmit.virus,general	TTVgen.	58°C	276	399.00	add
G1100	Transfusion transmitted.virus, types	TTV 1a,1b,2,3	60°C	415, …	399.00	add
G1110	Treponema pallidum	Tpa.	58°C	325	399.00	add
G1120	Trichomonas vaginalis	Tva.	58°C	771	399.00	add
G1130	Ureaplasma parvum, biovar 1	Upa.	58°C	540	399.00	add
G1140	Ureaplasma (parvum+ urealytic.)	Ure.	58°C	554	399.00	add
G1150	Ureaplasma urealyticum, biovar2	Uur.	58°C	435	399.00	add
G1160	Varicella-zoster virus	VZV	58°C	381	399.00	add
G1170	Vibrio cholerae (tox R)	Vcho (toxR)	58°C	624	399.00	add
G1180	Vibrio cholerae (îmð W)	Vcho (împW)	58°C	441	399.00	add
G1190	Yersinia enterocolitica	Yen	58°C	373	399.00	add
G1200	Yersinia pestis	Ype	58°C	351	399.00	add
G1210	Yersinia pseudotuberculesis	Yps	58°C	272	399.00	add

Please order/request the products by E-mail using this form: info@geneon.net

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