Restriction endonucleases are produced from an ISO 9001 certificated company in Europe. All Enzymes are supplied with a concentration of 10µ/µl.

Table of Restriction Endonucleases

Table of Isoschizomeres

Isosch.	Link to Enzyme	Isosch.	Link to Enzyme	Isosch.	Link to Enzyme	Isosch.	Link to Enzyme
Aat I	SseB I	BsiX	BseC I	Cbi I	Asu II	NgoM IV	Nae I
Acc III	BseA I	BIsp106 I	BseC I	Cfr42 I	SgrB I	NspV	Asu II
Afi II	MspC I	Bsp119 I	Asu II	Cla I	BseC I	PaeR7 I	Sla I
Age I	CspA I	Bsp13 I	BseA I	Csp45 I	Asu II	Pal I	BshF I
AsiA II	CspA I	BspD I	BseC I	Eco147 I	SseB I	PinA I	CspA I
Ban III	BseC I	BspE I	BseA I	EcoR II	BseB I	Pm55 II	SseB I
Bfr I	MspCI	BspT 1	MspC I	Hae III	BshF I	PspG I	BseB I
Bpu14 I	Asu II	BspX I	BseC I	Kpn I	BseA I	Sac II	SgrB I
Bsa29 I	BseC I	Bst2U I	BseB I	Ksp I	SgrB I	Sfr274 I	Sla I
Bsc I	BseC I	Bst98 I	MspC I	Lsp I	Asu II	Sfr303 I	SgrB I
Bsh I	BshF I	BstB I	Asu II	Mro I	BseA I	Sfu I	Asu II
Bsh T I	CspA I	BstN I	BseB I	MroN I	Nae I	Sst II	SgrB I
BsiC I	Asu II	BstO I	BseB I	Msp I	BsiS I	Stu I	SseB I
BsiL I	BseB I	Bsu15 I	BseC I	Mva I	BseB I	Tlil	Sla I
BsiM I	BseA I	BsuR I	BshF I	NgoA IV	Nae I	Xho I	SseB I

Quality Test Methods

Overnight Assay for Nuclease Contamination
GeneON uses an overnight assay as a qualitative determination of enzyme purity and of a lack of nonspecific DNases. In this assay, increasing amounts of the enzyme are added to a series of tubes containing 1 μg of substrate DNA (λ DNA/Hind III fragments or pBR322/Hinf I fragments). After a 20-hours incubation under the recommended assay conditions, the characteristic banding pattern of the DNA substrate is determined by agarose gel/ethidium bromide electrophoresis. A sharp, unaltered pattern under these conditions is an indication that the enzyme preparation is free of detectable levels of nonspecific DNases. The results of the assay are reported on the Technical Data Sheet provided with each enzyme.

Assay for Nonspecific Endonucleases
The assay is performed in 50 μl of the reaction mixture, containing increasing amounts of the enzyme, its assay buffer and 1 μg of a supercoiled plasmid substrate. After a 20-hours incubation under appropriate conditions the DNA is examined for structural alterations in 1% agarose gel. The endodeoxyribonuclease activity is expressed as a percent of supercoiled DNA converted to open circular or linear form. The results of the assay are reported on the Technical Data Sheet provided with each enzyme.

Stability
The enzymes are stored at -20°C. The expiry date of the enzymes is indicated in the product Technical Data Sheet and on the vial label.

Shipment
On blue Ice

GeneON guarantees the maintenance of quality of DNA/RNA modifying enzymes until their respective expiry dates.