Taq DNA Polymerase 2X-preMix

Maximo Tag Polymerase as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR

Cat.-no	Description	Amount	Price €	Shop
S113	Maximo Taq DNA Polymerase 2X-preMix	1x100 rcs (2x1.25 ml)	40.00	add
S114	Maximo Taq DNA Polymerase 2X-preMix	10x100 rcs (20x1.25 ml)	350.00	add
S114X	Maximo Taq DNA Polymerase 2X-preMix	other amounts	on request

Maximo Taq DNA Polymerase 2X-preMix:Order/request by E-mail:
Maximo Taq DNA Polymerase 2X-preMix: Datasheet
Maximo Taq DNA Polymerase 2X-preMix: Deutsche Beschreibung

Features:
Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit. The optimized mixture of all components reduces pipetting mistakes and ensures repeatable results - every day.

Applications:
- Standard / General PCR
- optimized for high specifity
- High-throughput PCR, automated pipetting, or plate based PCR
- Gene mutation
- T/A cloning

Description:
Maximo Taq DNA Polymerase 2X-preMix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template has to be added.
Maximo Taq DNA Polymerase 2X-preMix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration: the mixture is 2X concentrated

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

List of components:
0.1U/ul Taq DNA Polymerase, 0.4 mM dATP, 0.4 mM dGTP, 0.4 mM dCTP, 0.4 mM dTTP, 4 mM MgSO₄, 20 mM KCl, 16 mM (NH₄)₂SO₄, 20 mM Tris-HCl, pH8.8

Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination

Transportation: on blue ice

Storage: at -20°C for 24 months

Usage:

Components Volume per reaction

2X Taq mastermix 25 µl

Up-stream primer (10 µM stock) 0,5-2.5 µl

Down-stream primer (10 µM stock) 0.5-2,5 µl

Template DNA 0.1-15 ng/ml plasmid DNA
1-10 µg/ml genomic DNA

Sterile dest. Water (molecular grade) up to 50 µl total reaction volume

Note:
- vortex all solutions and spin down carefully before using
- dispense on ice and spin down again
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	1-5 min	94-95°C
25-30 Cycles: Denaturation Annealing Extension	10-25 sec 10-25 sec 60 sec	94-95°C 45-70°C 68-72°C per 1kb
Final extension	5 min	68-72°C

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