Taq DNA Polymerase blue ready-to-load

Maximo Tag-Blue Polymerase is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed!

Cat.-no	Description	Amount	Price €	Shop
S111	Maximo Taq-Blue DNA Polymerase	500 units	55.00	add
S112	Maximo Taq-Blue DNA Polymerase	5x500 units	269.00	add
S128	Maximo Taq-Blue DNA Polymerase	20x500 units	729.00	add
S129	Maximo Taq-Blue DNA Polymerase	100x500 units (or as bulk)	2259.00	add
S129X	Maximo Taq-Blue DNA Polymerase	other amounts	on request

Maximo Taq DNA Polymerase Blue: Order/request by E-mail
Maximo Taq-Blue DNA Polymerase: Datasheet
Maximo Taq-Blue DNA Polymerase: Deutsche Beschreibung

Features:
Maximo Taq-Blue DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. After PCR reaction the enzyme can be loaded to agarose gel, no dye and DNA loading buffer is needed. The enzyme is time- and cost saving, because it includes dye and loading buffer, already.

Applications:
- Standard / General PCR with visible control
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

Description:
Maximo Tag-Blue DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration: 1 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP 40, 0.5% Tween 20, in blue loading buffer

Reaction Buffers supplied with the enzyme:

10X Buffer I: 500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂
MgCl₂: 100 mM

Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2,2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination

Transportation: on blue ice

Storage: at -20°C for 12 months

Usage:

Components Volume per reaction

10X reaction buffer I or buffer II 5 µl

25 mM MgCl₂ 1.5 µl (if you use buffer II)

dNTP-Mix (40mM) 1.0 µl

Up-stream primer (10 µM stock) 0,5-2.5 µl

Down-stream primer (10µM stock) 0.5-2,5 µl

Template DNA 0.1-15 ng/ml plasmid DNA
1-10 µg/ml genomic DNA

Maximo Taq DNA-Blue Polymerase (1 u/µl) 0.8 µl - 2 µl

Sterile dest. Water (molecular grade) up to 50 µl total reaction volume

Note:
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	2-5 min	94-95°C
25-30 Cycles: Denaturation Annealing Extension	10-25 sec 10-25 sec 60 sec	94-95°C 55-65°C 72°C per 1kb
Final extension	5 min	72°C

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